TRANSPORT OF GLUTATHIONE S-CONJUGATES IN THE YEASTSSACCHAROMYCES CEREVISIAE |
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Authors: | ROLAND ŻĄDZIŃSKI JANUSZ MASZEWSKI GRZEGORZ BARTOSZ |
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Affiliation: | 1. Department of Cytophysiology, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland;2. Department of Molecular Biophysics, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland |
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Abstract: | Transport of 2,4-dinitrophenyl-S-glutathione (DNP-SG) and a fluorescent glutathione S-conjugate, bimane-S-glutathione (B-SG) was studied in the baker's yeasts (S. cerevisiae). Both conjugates were exported from the cells; the transport was inhibited by fluoride and vanadate like in mammalian cells. B-SG was also found to be accumulated in the vacuoles. The transport rate of DNP-SG outside the cell was higher in a vacuolar-deficient strain. A significant ATP-dependent uptake of (3H)-DNP-SG by vacuoles was found. These results indicate thatS. cerevisiaetransport glutathione S-conjugates both outside the cells and into the vacuoles. |
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Keywords: | yeasts Saccharomyces cerevisiae glutathione S-conjugates membrane transport |
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