Kinetic characteristics of long‐term repeated fed‐batch (LtRFb) l‐lactic acid fermentation by a Bacillus coagulans strain |
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Authors: | Fan Zhang Jiongqin Liu Xiao Han Chao Gao Cuiqing Ma Fei Tao Ping Xu |
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Institution: | 1. State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai P. R. China ; 2. State Key Laboratory of Microbial Technology, Shandong University, Qingdao P. R. China |
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Abstract: | Application of degradable plastics is the most critical solution to plastic pollution. As the precursor of biodegradable plastic PLA (polylactic acid), efficient production of l‐lactic acid is vital for the commercial replacement of traditional plastics. Bacillus coagulans H‐2, a robust strain, was investigated for effective production of l‐lactic acid using long‐term repeated fed‐batch (LtRFb) fermentation. Kinetic characteristics of l‐lactic acid fermentation were analyzed by two models, showing that cell‐growth coupled production gradually replaces cell‐maintenance coupled production during fermentation. With the LtRFb strategy, l‐lactic acid was produced at a high final concentration of 192.7 g/L, on average, and a yield of up to 93.0% during 20 batches of repeated fermentation within 487.5 h. Thus, strain H‐2 can be used in the industrial production of l‐lactic acid with optimization based on kinetic modeling. |
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Keywords: | Bacillus coagulans Kinetics l‐ Lactic acid Long‐ term repeated fed‐ batch |
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