OCCURRENCE OF PHYIWATED CHLOROPHYLL c IN ISOCHRYSIS GALBANA AND ISOCHRYSIS SP. (CLONE T-ISO) (PRYMNESIOPHYCEAE)1 |
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Authors: | Manuel Zapata José L. Garrido |
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Affiliation: | Instituto de Investigacions Mariñas, CSIC, Av. Eduardo Cabello 6, 36208 Vigo, Spain |
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Abstract: | The pigment composition of two clones of Isochrysis galbana Parke (CCMP 1323 and CCAP 927/1), and Isochrysis sp. (clone T-ISO) was analyzed by high-performance liquid chromatography using a polymeric octadecylsilica column. Fluorescent peaks with retention times higher than chlorophyll a were detected for all three clones. The corresponding pigments were isolated and characterized in terms of their visible absorbance and fluorescence spectra. The pigments were similar to phytol-substituted chlorophyll c, previously isolated from Emiliania huxleyi (Lohm.) Hay and Mohler and other species containing chlmophyll c3. The presence of phytol-substituted chlorophyll c in I. galbana which lacked chlorophyll c3, increases the diversity of chlorophyll patterns for the Haptophyta, which can be grouped, at present, into six different pigment types. This is the jrst observation of a haptophyte containing the apolar phytylated chlorophyll c-like pigment but lacking chlorophyll c3. |
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Keywords: | Key index words: Haptophyta HPLC Isochrysidiales Isochrysis galbana Isochrysis sp. (clone T-ISO) phytylated chlorophyll c—like pigment polymeric octadecylsilica columns Prymnesiophyceae |
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