Somaclonal variation in rye (<Emphasis Type="Italic">Secale cereale</Emphasis> L.) analyzed using polymorphic and sequenced AFLP markers |
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Authors: | Raúl de la Puente Ana Isabel González María Luisa Ruiz Carlos Polanco |
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Institution: | (1) área de Genética, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, 24071 León, Spain |
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Abstract: | Amplified fragment length polymorphism (AFLP) analysis of 24 in vitro regenerated rye plants was performed in order to evaluate the somaclonal variation rate in this species and to identify rye
genomic regions where mutations are preferentially promoted by in vitro culture processes. Regenerated plants were obtained from cell lines derived from immature embryos and plants were regenerated
by somatic embryogenesis. Twenty-three regenerants showed variation when compared against sibling plants obtained from the
same cell line. A total number of 887 AFLP markers were scored, and 8.8% identified the same polymorphism in plants obtained
independently from different cell lines, revealing putative mutational hot spots. Using controlled crossings and analysis
of the corresponding progenies, we were able to verify the genetic stability in the next generation for only five of these
polymorphisms. The nucleotide sequence of the AFLP amplicon of four of the polymorphic markers was obtained, but only the
sequence of two markers was clearly identified in the databases. The sequence of marker A1-303 was identified as part of a
tandemly repeated sequence, the 120-bp family, which is located at telomeric regions and is widely distributed among rye chromosomes.
The marker A5-375 showed high similarity with regions of Angela retrotransposons. |
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Keywords: | Somaclonal variation AFLP analysis Secale cereale |
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