PCR analysis to distinguish between alleles of a member of a multigene family correlated with wheat bread-making quality |
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| Authors: | R D'Ovidio O D Anderson |
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| Institution: | (1) Dipartimento di Agrobiologia e Agrochimica, Universita degli Studi della Tuscia, via S, Camillo de Lellis, 01100 Viterbo, Italy;(2) U.S. Department of Agriculture, Western Regional Research Center, 800 Buchanan Street, 94710 Albany, CA, USA;(3) Present address: Dipartimento di Agrobiologia e Agrochimica, Universita degli Studi della Tuscia, via S, Camillo de Lellis, 01100 Viterbo, Italy |
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| Abstract: | Good or poor wheat bread-making quality is associated with two allelic pairs at theGlu-D1 complex locus, designated 1Dx5-1Dy10 and 1Dx2-1Dy12, respectively. The polymerase chain reaction (PCR) verified the presence of the HMW-glutenin 1Dx5 gene from genomic DNA extracted from part of the endosperm of a single dry seed, or a small amount of leaf or root tissue, of several bread-wheat cultivars. This easy, quick, and non-destructive PCR-based approach is proposed as a very efficient and safe alternative to standard procedures for selecting bread-wheat genotypes with good bread-making properties. |
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| Keywords: | Wheat HMW-glutenin genes Bread-making quality Polymerase Chain Reaction(PCR) Multigene families |
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