人OC-3-VGH卵巢癌细胞裸小鼠肿瘤模型的建立

目的建立人OC-3-VGH细胞株卵巢癌裸小鼠模型并观察该肿瘤生物学生长特性。方法OC-3-VGH细胞株复苏后加入10 mL RPMI-1640培养液,放入培养箱,传2～3代后,取细胞悬液,均以4×106个细胞,每只0.2 mL分别接种至BALB/c雌性裸小鼠皮下,2月后处死取材,观察肿瘤生长特性和转移情况。结果皮下接种一周后,裸鼠长出肿瘤,并随时间而增大,体积呈指数增长,第42天始,明显增大（P〈0.05）。组织学检查发现裸鼠皮下肿瘤细胞均细胞体积较大,细胞核大而染色深,核分裂相较多、异型性明显,接种2个月时,未发生其他组织转移。结论建立了新的卵巢癌动物模型,并初步研究了其生物学特性,为卵巢癌治疗方法的研究拓宽了道路。

Establishmet of a Nude Mouse Model of Ovarian Cancer with a Human Ovarian Cancer OC-3-VGH Cell Line

Objective To establish a nude mouse model of ovarian cancer with a human ovarian cancer OC-3-VGH cell line and study its biological characteristics.Methods The OC-3-VGH cells were moved into new culture flasks added with 10 mL RPMI-1640 nutrient solution and put into incubator,with refreshed culture medium on the next day.Cell suspension of 4×106 cells/ 0.2 mL was injected into BALB/c female nude mice subcutaneously.All mice were sacrificed after 2 months.Results All the ovarian tumors in the nude mice became discernible one week after hypodermic inoculation,and distinctly bigger after the 42th day（P〈0.05）.Histological examination revealed that the tumor cells were large and stained deeply,without metastasis at two months after inoculation.Conclusion A novel nude mouse model of ovarian cancer has been successfully established and it offers a new method for research of this disease.