The phorbol ester TPA enhances A23187--but not carbachol- and high K+-induced catecholamine secretion from cultured bovine adrenal chromaffin cells |
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| Authors: | K Morita K W Brocklehurst S M Tomares H B Pollard |
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| Affiliation: | 1. Department of Cardiovascular Medicine, First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China;2. Smidt Heart Institute, Department of Cardiology, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA;3. Institute for Functional Genomics, University of Montpellier, CNRS, INSERM, Montpellier, France;4. Department of Physiology, UCLA, Los Angeles, CA 90095, USA;5. Department of Anesthesiology and Perioperative Medicine, Division of Molecular Medicine, UCLA, Los Angeles, CA 90095, USA;1. Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, New York, NY, 10010, USA;2. Biology Program, Division of Science and Mathematics, New York University Abu Dhabi, Abu Dhabi, United Arab Emirates;1. Departments of Physiology and Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA;2. Department of Biomedical Sciences and Public Health, University “Politecnica Delle Marche”, Via Tronto 10/A, 60126, Ancona, Italy;3. Department of Physiology and Pharmacology, Sackler School of Medicine, Tel-Aviv University, 69978, Israel;4. IRCSS SDN, Via Gianturco, 80131, Naples, Italy;5. Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, M13 9PT, UK;6. Achucarro Centre for Neuroscience, IKERBASQUE, Basque Foundation for Science, 48011, Bilbao, Spain |
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| Abstract: | The effect of the phorbol ester TPA on catecholamine secretion was studied in cultured bovine adrenal chromaffin cells. The pretreatment of chromaffin cells with TPA caused the enhancement of catecholamine secretion induced by the calcium ionophore, A23187. By contrast, neither carbachol- nor high K+-induced secretion was changed by TPA pretreatment. These results support the concept that protein kinase C plays an important role as a factor transducing the Ca2+ signal to the exocytotic process of catecholamine secretion in bovine adrenal chromaffin cells. |
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