Putative nuclease-sensitive control element in unfertilized eggs of the sea urchin Lytechinus pictus |
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| Authors: | E N Mandley A C Lopo |
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| Institution: | 1. Department of Baccalaureate Biology, St. Petersburg College, Clearwater, FL, United States;2. Department of Molecular, Cellular and Developmental Biology and Marine Science Institute, UC Santa Barbara, Santa Barbara, CA, United States;3. Department of Molecular and Cell Biology and Biochemistry, Brown University, Providence, RI, United States;1. Université Côte d''Azur, CNRS, INSERM, iBV, Nice, France;2. Université Pierre et Marie Curie, Observatoire Océanologique de Villefranche sur Mer, UMR7009 CNRS, Paris, France |
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| Abstract: | Very limited nuclease-treatment of actively translating lysates from unfertilized eggs of the sea urchin Lytechinus pictus results in activation of the lysate. This activation is reflected by the ability of the nuclease-treated (or 'nuclease-activated') lysates to more efficiently utilize exogenous added RNAs. The activation may be reversed by adding small aliquots of untreated lysate to the nuclease-activated lysates. These results suggest that a nuclease-sensitive negative control element may be involved in the translational repression of unfertilized eggs, and that a deactivation of this control element is partially responsible for the dramatic activation of protein synthesis following fertilization in sea urchin eggs. |
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