Incompatibility and molecular relationships between small staphylococcal plasmids carrying the same resistance marker |
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Authors: | S Iordanescu Marietta Surdeanu Phyllis Della Latta Richard Novick |
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Institution: | 1. Institute Cantacuzino, Bucharest, Rumania;2. The Public Health Research Institute of The City of New York Inc., New York, New York 10016 U.S.A. |
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Abstract: | Incompatibility relationships between staphylococcal plasmids carrying the same, single resistance marker were studied by means of appropriate recombinant plasmids. Naturally occurring plasmids encoding streptomycin, tetracycline, or chloramphenicol resistance, respectively, were used in this study, four of each phenotype. The plasmids responsible for tetracycline resistance proved to belong to a single incompatibility set. Similarly, the four streptomycin resistance plasmids fall in the same incompatibility set. On the other hand, plasmids encoding chloramphenicol resistance were divided in four distinct incompatibility sets, three of them being newly defined. Study of the molecular relationships between these plasmids by DNA-DNA hybridization and restriction endonuclease cleavage supported the conclusions from genetic tests that the four Tcr and the four Smr plasmids are essentially identical, whereas the four Cmr plasmids are diverse. |
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