High xylosyltransferase activity in children and during mineralization of osteoblast-like SAOS-2 cells |
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Authors: | Christian Prante Joachim Kuhn Knut Kleesiek Christian Götting |
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Institution: | 1.Institut für Laboratoriums- und Transfusionsmedizin, Herz- und Diabeteszentrum NRW,Universit?tsklinik der Ruhr-Universit?t Bochum,Bad Oeynhausen,Germany |
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Abstract: | Skeletal growth and tissue remodelling processes are characterized by an elevated collagen and proteoglycan biosynthesis.
The xylosyltransferases I and II are the rate-limiting step enzymes in proteoglycan biosynthesis and serum xylosyltransferase
(XT) activity has been shown to be a biomarker for the actual proteoglycan biosynthesis rate. Here, XT, alkaline phosphatase
(ALP), bone ALP (BALP) activities were measured in 133 juvenile Caucasians. Serum XT activities in juveniles were elevated
and significantly correlated with ALP and BALP. In an osteoblast-like cell model using SAOS-2 cells mineralization and bone
nodule formation were induced and XT-I, XT-II and ALP were monitored. Induction of mineralization in SAOS-2 cells resulted
in a long-term increase of XT-I mRNA and enzyme activity, which could be paralleled with elevated ALP activity. In addition,
HGH and IGF-I treatment of SAOS-2 cells led to an increased expression of XT-I and ALP. These results point to skeletal growth
and tissue remodeling as a cause of the high XT activity in children. |
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Keywords: | Xylosyltransferase Alkaline phosphatase Osteogenesis Proteoglycan Glycosaminoglycans |
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