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| 耐盐偶氮染料脱色菌株GYW的筛选及特性 | |
| 引用本文： | 郭建博,张立辉,杨景亮,洪煜斌,刘 春,李再兴,王晓磊,康 丽.耐盐偶氮染料脱色菌株GYW的筛选及特性[J].微生物学通报,2009,36(5):0644-0651. |
| 作者姓名： | 郭建博 张立辉 杨景亮 洪煜斌 刘 春 李再兴 王晓磊 康 丽 |
| 作者单位： | 1. 河北科技大学,环境科学与工程学院,河北,石家庄,050018;三达膜科技,厦门,科技有限公司,福建,厦门,361022 2. 河北科技大学,环境科学与工程学院,河北,石家庄,050018 3. 三达膜科技,厦门,科技有限公司,福建,厦门,361022 |
| 基金项目： | 河北省自然科学基金(No. E200700063) |
| 摘 要： | 从某印染厂排水沟的底泥中分离筛选到1株对偶氮染料具有脱色能力的耐盐菌株GYW, 经16S rDNA序列分析, 鉴定为盐单胞菌属(Halomonas)中度耐盐菌。实验结果表明, 菌株GYW可以耐受10%以上的高盐度, 对酸性大红GR和其它偶氮染料具有广谱的脱色能力, 处于对数生长期的细胞脱色能力最强。对酸性大红GR的最佳脱色条件为：温度30°C, pH 7.5, LB培养基。氯离子对酸性大红GR脱色的抑制作用较强, 硫酸盐对脱色影响不大, 添加甜菜碱可提高染料的脱色速率, 最佳添加量为200 mg/L。 |
| 关 键 词： | 耐盐菌 偶氮染料 脱色 甜菜碱 |
Isolation and Characteristic of an Azo Dye-decolorizing Salt-tolerant Bacteria Strain GYW |
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| GUO Jian-Bo,ZHANG Li-Hui,YANG Jing-Liang,HONG Yu-Bin,LIU Chun,LI Zai-Xing,WANG Xiao-Lei and KANG Li.Isolation and Characteristic of an Azo Dye-decolorizing Salt-tolerant Bacteria Strain GYW[J].Microbiology,2009,36(5):0644-0651. | |
| Authors: | GUO Jian-Bo ZHANG Li-Hui YANG Jing-Liang HONG Yu-Bin LIU Chun LI Zai-Xing WANG Xiao-Lei and KANG Li |
| Institution: | School of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China; Suntar Membrane Technology (Xiamen) Co., Ltd., Xiamen, Fujian 361022, China;School of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China;School of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China;Suntar Membrane Technology (Xiamen) Co., Ltd., Xiamen, Fujian 361022, China;School of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China;School of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China;School of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China;School of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China |
| Abstract: | A salt-tolerant strain GYW capable of decolorating azo dye was isolated and identified as Halomonas sp. by 16S rDNA. The result showed that the decolorizing salt-tolerant bacteria could survive above the 10% salt concentration and it could decolorize many dyes. The strain had a high decolorizing rate on acid red GR under the condition of pH 7.5, anaerobic 30°C and 10% NaCl. The ion of Cl- strong inhibited the decolorization of acid red GR, and the ion of SO42- affected little on the decolorization, and the lycine addition with the optimal concentration of 200 mg/L could enhanced the decolorization rate under high NaCl concentration. |
| Keywords: | Salt-tolerant bacteria Azo dye Decolorization Lycine |
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