A rapid method to screen fungi for resistance to toxic chemicals |
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Authors: | Bruce C. Alleman Bruce E. Logan Robert L. Gilbertson |
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Affiliation: | (1) Environmental Technology Department, Battelle Memorial Institute, 43201-2693 Columbus, Ohio, USA;(2) Environmental Engineering Program, Department of Civil Engineering and Engineering Mechanics, University of Arizona, 85721 Tucson, AZ, USA;(3) Department of Plant Pathology, University of Arizona, 85721 Tucson, AZ, USA |
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Abstract: | Although many species of fungi are able to degrade highly toxic chemicals, only a few species have been evaluated for resistance to toxic effects of these chemicals. In this paper we demonstrate the successful application of a method to rapidly screen several species of fungi for toxicity to chemicals or mixtures of chemicals using pentachlorophenol (PCP) as a model toxic compound. Cellulose antibiotic assay disks were soaked in solutions containing different concentrations of PCP (5, 10, 25, 50, and 80 mg l–1) and then placed in a triangular pattern outside the growing edge of the mycelia of eighteen species of white rot fungi. The plates were incubated and observed for development of inhibition zones (non-growth areas) around the disks. The short-term (24 h) growth of all eighteen species of fungi was inhibited by 5–10 mg-PCP l–1, a range similar to that observed using previously reported techniques. Long-term growth studies using this screening method were not useful since PCP diffused from the disk into the agar, decreasing the applied dose. |
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Keywords: | chemical toxicity fungi pentachlorophenol biodegradation |
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