A colorimetric assay for gellan elaborated by Sphingomonas paucimobilis ATCC 31461 |
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| Authors: | West T.P. Strohfus B. Santiago M.F. |
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| Affiliation: | (1) Olson Biochemistry Laboratories, Department of Chemistry and Biochemistry, South Dakota State University, Brookings, SD 57007, USA |
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| Abstract: | A colorimetric assay involving the dye toluidine blue O was developed to determine the concentration of the microbial heteropolysaccharide gellan elaborated by Sphingomonas paucimobilis ATCC 31461. Colour formation was linear up to a concentration of 0.7 mg/ml. The concentration of gellan produced in S. paucimobilis cultures was quantitated using this colorimetric dye-binding assay as well as the currently utilized gravimetric procedure, and comparable results were observed. This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | Colorimetric gellan heteropolysaccharide Sphingomonas toluidine blue O |
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