Growth of wool follicles in culture |
| |
Authors: | J J Bond P C Wynn G N Brown G P M Moore |
| |
Institution: | (1) Department of Animal Science, University of Sydney, Werombi Rd., 2570 Camden, NSW, Australia;(2) Biometrics Unit, Institute of Animal Production and Processing, CSIRO, P.O. Box 239, 2148 Blacktown, NSW, Australia;(3) Division of Animal Production, CSIRO, P. O. Box 239, 2148 Blacktown, NSW, Australia |
| |
Abstract: | A procedure for the culture of isolated wool follicles from Merino sheep is described. Follicles were microdissected from
midside skin samples of 2-yr-old wethers and transferred, individually, to 24-well tissue culture plates. When maintained
in supplemented Williams’ E medium containing 5 to 10% fetal bovine serum (FBS), insulin, hydrocortisone, and a trace element
mixture, fiber growth rates of 40 to 80 μm/day were observed. Follicles maintained their morphologic integrity for up to 7
days, incorporated methyl-3H]thymidine into DNA and 35S]methionine into intermediate-filament keratins of the growing fiber. Insulin and hydrocortisone stimulated fiber growth
at concentrations of 10 μg/ml and 50 ng/ml, respectively, but higher doses were inhibitory. The growth of fibers in response
to hydrocortisone and the changes in follicle morphology was similar to those induced in skin after systemic administration
of cortisol in vivo. A positive interaction between hydrocortisone and trace elements for follicle survival and hydrocortisone,
insulin, and FBS for fiber growth was also found. The successful culture of Merino sheep follicles provides a model with which
to study the direct influence of endocrine, nutritional and local factors on wool keratin synthesis independently of systemic
shifts in the animals’ metabolism. |
| |
Keywords: | wool follicle fiber growth culture |
本文献已被 SpringerLink 等数据库收录! |
|