| Abstract: | SYNOPSIS. Protostelium was cultured monoxenically with the yeast Rhodotorula mucilaginosa on either corn meal agar or in liquid corn meal medium. Nearly synchronous encystment was induced in amoebae by washing them free of yeast and incubating them in an encystment medium devised by Neff et al. (1964) for Acanthamoeba.
The extrinsic requirements for encystment were investigated by replacing various components of the encystment medium with other substances. The results indicate that encystment depends on a high concentration of inorganic monovalent cations and that it occurs best at a basic pH. The effect of the ions does not appear to be strictly osmotic. |
