Physiological concentrations of ADP stimulate the release of prostacyclin from bovine aortic endothelial cells |
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Authors: | A Van Coevorden JM Boeynaems |
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Institution: | Institute of Interdisciplinary Research and Department of Endocrinology of Erasme Hospital, Université Libre de Bruxelles, B-1070 Brussels, Belgium |
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Abstract: | ADP (0.2−200 μN) stimulated the synthesis of prostacyclin (PGI2), as reflected by the release of 6-keto-prostaglandin F1α (6-K-PGF1α), in endothelial cells cultured from bovine orta. This effect of ADP was mimicked by ATP, whereas AMP and adenosine were completely inactive. The release of 6-K-PGF1α triggered by ADP was rapid in onset (within 5 min), transient (10 min) and followed by a period of refractoriness to a new ADP challenge. Growing and confluent cells were equally responsive to ADP. ADP stimulated the release of free arachidonic acid from the endothelial cells. ADP could be thus exert two opposite actions on platelet aggregation in vivo: a direct stimulation and an inhibition mediated by PGI2. This last action might contribute to limit thrombus formation to areas of endothelial cell damage. |
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