Synthesis and turnover of collagen precursors in rabbit skin |
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| Authors: | Marcel E. Nimni Ersilia De Guia Lucien A. Bavetta |
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| Affiliation: | Department of Biochemistry and Nutrition, School of Dentistry, University of Southern California, Los Angeles, Calif., U.S.A. |
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| Abstract: | 1. The rate of synthesis of [(14)C]hydroxyproline by rabbit skin was studied in vitro and in vivo. 2. The soluble collagen fractions were shown to have a very rapid turnover. The 0.15m-sodium chloride-extractable collagen showed t((1/2)) values of 1.2hr. in vitro and 12hr. in vivo. The 0.5m-sodium chloride-extractable collagen exhibited a t((1/2)) value of 20hr. in vivo. 3. Under the conditions used it was not possible to obtain radioactive insoluble collagen in vitro. 4. A significant amount of soluble collagen is lost before it becomes insoluble. 5. These observations may help to explain why large amounts of peptide-bound hydroxyproline appear in the urine during periods of rapid collagen synthesis. |
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