Colorimetric detection of bisphenol A based on unmodified aptamer and cationic polymer aggregated gold nanoparticles |
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| Authors: | Dongwei Zhang Jiayi Yang Jing Ye Lurong Xu Hanchu Xu Shenshan Zhan Bing Xia Lumei Wang |
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| Affiliation: | 1. School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, China;2. School of Agriculture and Biology and Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, and Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai 200240, China;3. Department of Management, Shanghai Sanda College, Shanghai 201209, China |
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| Abstract: | In this study, a colorimetric method was exploited to detect bisphenol A (BPA) based on BPA-specific aptamer and cationic polymer-induced aggregation of gold nanoparticles (AuNPs). The principle of this assay is very classical. The aggregation of AuNPs was induced by the concentration of cationic polymer, which is controlled by specific recognition of aptamer with BPA and the reaction of aptamer and cationic polymer forming “duplex” structure. This method enables colorimetric detection of BPA with selectivity and a detection limit of 1.50 nM. In addition, this colorimetric method was successfully used to determine spiked BPA in tap water and river water samples. |
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| Keywords: | BPA, bisphenol A HPLC, high-performance liquid chromatography ssDNA, single-stranded DNA AuNP, gold nanoparticle PDDA, poly(diallyldimethylammonium chloride) E2, 17β-estradiol PRG, progesterone THI, thiamphenicol o,p'-DDT, o,p'-dichlorodiphenyltrichloroethane DES, diethylstilbestrol 7-ACA, 7-aminocephalosporanic acid Kana, kanamycin Gly, glycine Cys, cysteine Vc, l-ascorbic acid Na3C6H5O7· 2H2O, trisodium citrate dehydrate DMSO, dimethyl sulfoxide AMP, ampicillin PBS, phosphate-buffered saline PCS, photon correlation spectroscopy CD, Circular dichroism Mix. 1, mixture 1 Mix. 2, mixture 2 |
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