Characterization of protein expression levels with label-free detected reverse phase protein arrays |
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| Authors: | Xuexue Guo Yihong Deng Chenggang Zhu Junlong Cai Xiangdong Zhu James P Landry Fengyun Zheng Xunjia Cheng Yiyan Fei |
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| Institution: | 1. Department of Optical Science and Engineering, Shanghai Engineering Research Center for Ultra-Precision Optical Manufacturing, Green Photoelectron Platform, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Fudan University, Shanghai, 200433, China;2. Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China;3. Department of Physics, University of California, Davis, CA, 95616, USA;4. Institutes of Biomedical Science, Fudan University, Shanghai, 200032, China |
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| Abstract: | In reverse-phase protein arrays (RPPA), one immobilizes complex samples (e.g., cellular lysate, tissue lysate or serum etc.) on solid supports and performs parallel reactions of antibodies with immobilized protein targets from the complex samples. In this work, we describe a label-free detection of RPPA that enables quantification of RPPA data and thus facilitates comparison of studies performed on different samples and on different solid supports. We applied this detection platform to characterization of phosphoserine aminotransferase (PSAT) expression levels in Acanthamoeba lysates treated with artemether and the results were confirmed by Western blot studies. |
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| Keywords: | Reverse phase protein arrays Label-free detection Quantification Normalization |
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