Bacteriophage immobilized graphene electrodes for impedimetric sensing of bacteria (Staphylococcus arlettae) |
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Authors: | Neha Bhardwaj Sanjeev K Bhardwaj Jyotsana Mehta Girish C Mohanta Akash Deep |
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Institution: | 1. Central Scientific Instruments Organisation (CSIR–CSIO), Chandigarh 160030, India;2. Academy of Scientific and Innovative Research, CSIR–CSIO, Chandigarh 160030, India |
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Abstract: | Bacteriophages are a class of viruses that specifically infect and replicate within a bacterium. They possess inherent affinity and specificity to the particular bacterial cells. This property of bacteriophages makes them an attractive biorecognition element in the field of biosensor development. In this work, we report the use of an immobilized bacteriophage for the development of a highly sensitive electrochemical sensor for Staphylococcus arlettae, bacteria from the pathogenic family of coagulase-negative staphylococci (CNS). The specific bacteriophages were covalently immobilized on the screen-printed graphene electrodes. Thus, the fabricated bacteriophage biosensor displayed quantitative response for the target bacteria (S. arlettae) for a broad detection range (2.0–2.0 × 106 cfu). A fast response time (2 min), low limit of detection (2 cfu), specificity, and stability over a prolonged period (3 months) are some of the important highlights of the proposed sensor. The practical utility of the developed sensor has been demonstrated by the analysis of S. arlettae in spiked water and apple juice samples. |
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Keywords: | Bacteriophage Graphene Impedimetric sensor Bacteria S   arlettae |
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