Biomolecular interaction analysis for carbon nanotubes and for biocompatibility prediction |
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Authors: | Xiaoping Chen Jinzhang Fang Yun Cheng Jianhui Zheng Jingjing Zhang Tao Chen Benfang Helen Ruan |
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Institution: | 1. College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China;2. College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China |
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Abstract: | The interactions between carbon nanotubes (CNTs) and biologics have been commonly studied by various microscopy and spectroscopy methods. We tried biomolecular interaction analysis to measure the kinetic interactions between proteins and CNTs. The analysis demonstrated that wheat germ agglutinin (WGA) and other proteins have high affinity toward carboxylated CNT (f-MWCNT) but essentially no binding to normal CNT (p-MWCNT). The binding of f-MWCNT–protein showed dose dependence, and the observed kinetic constants were in the range of 10−9 to 10−11 M with very small off-rates (10−3 to 10−7 s−1), indicating a relatively tight and stable f-MWCNT–protein complex formation. Interestingly in hemolysis assay, p-MWCNT showed good biocompatibility, f-MWCNT caused 30% hemolysis, but WGA-coated f-MWCNT did not show hemolysis. Furthermore, the f-MWCNT–WGA complex demonstrated enhanced cytotoxicity toward cancer cells, perhaps through the glycoproteins expressed on the cells' surface. Taken together, biomolecular interaction analysis is a precise method that might be useful in evaluating the binding affinity of biologics to CNTs and in predicting biological actions. |
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Keywords: | Carbon nanotubes Biomolecular interaction analysis Biocompatibility Hemolysis Modified biomaterials |
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