When alcohol is the answer: Trapping,identifying and quantifying simple alkylating species in aqueous environments |
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Authors: | Philip G. Penketh Krishnamurthy Shyam Raymond P. Baumann Rui Zhu Kimiko Ishiguro Alan C. Sartorelli Elena S. Ratner |
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Affiliation: | 1. Department of Obstetrics, Gynecology & Reproductive Sciences, Yale University School of Medicine, New Haven, CT 06520-8063, USA;2. Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06520-8066, USA |
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Abstract: | Alkylating agents are a significant class of environmental carcinogens as well as commonly used anticancer therapeutics. Traditional alkylating activity assays have utilized the colorimetric reagent 4-(4-nitrobenzyl)pyridine (4NBP). However, 4NBP based assays have a relatively low sensitivity towards harder, more oxophilic alkylating species and are not well suited for the identification of the trapped alkyl moiety due to adduct instability. Herein we describe a method using water as the trapping agent which permits the trapping of simple alkylating electrophiles with a comparatively wide range of softness/hardness and permits the identification of donated simple alkyl moieties. |
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Keywords: | Alkylating Chemotherapy Carcinogen Laromustine Nitrosoureas Sulfonylhydrazines |
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