The effect of explosive blast loading on human neuroblastoma cells |
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| Authors: | Nicole E. Zander Thuvan Piehler Rohan Banton Mary Boggs |
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| Affiliation: | 1. U.S. Army Research Laboratory, Weapons and Materials Research Directorate, Aberdeen Proving Ground, MD 21005, USA;2. Department of Biology, University of Delaware, Newark, DE 19716, USA |
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| Abstract: | Diagnosis of mild to moderate traumatic brain injury is challenging because brain tissue damage progresses slowly and is not readily detectable by conventional imaging techniques. We have developed a novel in vitro model to study primary blast loading on dissociated neurons using nitroamine explosives such as those used on the battlefield. Human neuroblastoma cells were exposed to single and triple 50-psi explosive blasts and single 100-psi blasts. Changes in membrane permeability and oxidative stress showed a significant increase for the single and triple 100-psi blast conditions compared with single 50-psi blast and controls. |
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| Keywords: | TBI, traumatic brain injury PBS, phosphate-buffered saline HBSS, Hank's balanced salt solution DMEM, Dulbecco's modified Eagle's medium FBS, fetal bovine serum P/S, penicillin/streptomycin Carboxy-H2DCFDA, 6-carboxy-2&prime ,7&prime -dichlorodihydrofluorescein diacetate ROS, reactive oxygen species CLSM, confocal laser scanning microscopy |
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