A robust method to screen detergents for membrane protein stabilization,revisited |
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Authors: | Philippe Champeil,Sté phane Orlowski,Simon Babin,Sten Lund,Marc le Maire,Jesper Mø ller,Guillaume Lenoir,Cé dric Montigny |
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Affiliation: | 1. Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, 91198 Gif-sur-Yvette, France;2. Medical Research Laboratory, Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus, Denmark;3. Centre for Membrane Pumps in Cells and Disease – PUMPKIN, Danish National Research Foundation, Aarhus University, 8000 Aarhus, Denmark;4. Department of Biomedicine, Aarhus University, 8000 Aarhus, Denmark |
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Abstract: | This report is a follow up of our previous paper (Lund, Orlowski, de Foresta, Champeil, le Maire and Møller (1989), J Biol Chem 264:4907–4915) showing that solubilization in detergent of a membrane protein may interfere with its long-term stability, and proposing a protocol to reveal the kinetics of such irreversible inactivation. We here clarify the fact that when various detergents are tested for their effects, special attention has of course to be paid to their critical micelle concentration. We also investigate the effects of a few more detergents, some of which have been recently advertised in the literature, and emphasize the role of lipids together with detergents. Among these detergents, lauryl maltose neopentyl glycol (LMNG) exerts a remarkable ability, even higher than that of β-dodecylmaltoside (DDM), to protect our test enzyme, the paradigmatic P-type ATPase SERCA1a from sarcoplasmic reticulum. Performing such experiments for one's favourite protein probably remains useful in pre-screening assays testing various detergents. |
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Keywords: | Detergent Membrane protein Lipid Stability LMNG SERCA1a |
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