An ultrasensitive electrochemical genosensor for Brucella based on palladium nanoparticles |
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Authors: | A Rahi N Sattarahmady H Heli |
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Institution: | 1. Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran;2. Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran |
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Abstract: | Palladium nanoparticles were potentiostatically electrodeposited on a gold surface at a highly negative potential. The nanostructure, as a transducer, was utilized to immobilize a Brucella-specific probe and the process of immobilization and hybridization was detected by electrochemical methods. The proposed method for detection of the complementary sequence and a non-complementary sequence was applied. The fabricated genosensor was evaluated for the assay of the bacteria in the cultured and human samples with and without PCR. The genosensor could detect the complementary sequence with a sensitivity of 0.02 μA dm3 mol−1, a linear concentration range of 1.0 × 10−12 to 1.0 × 10−19 mol dm−3, and a detection limit of 2.7 × 10−20 mol dm−3. |
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Keywords: | Palladium Nanoparticles Genosensor Brucella DNA biosensor |
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