| 导入外源DNA小麦变异类型的光合特性研究(简报) |
| |
| 引用本文： | 王晓娟,孔英珍,王亚馥,庞斌双,倪建福.导入外源DNA小麦变异类型的光合特性研究(简报)[J].分子细胞生物学报,2000,33(2):195-198. |
| |
| 作者姓名： | 王晓娟 孔英珍 王亚馥 庞斌双 倪建福 |
| |
| 作者单位： | 王晓娟（兰州大学干旱农业生态国家重点实验室，兰州730000）;孔英珍（兰州大学干旱农业生态国家重点实验室，兰州730000）;王亚馥（兰州大学干旱农业生态国家重点实验室，兰州730000）;庞斌双（甘肃省农科院，兰州730070）;倪建福（甘肃省农科院，兰州730070） |
| |
| 基金项目： | 甘肃省农业重点科技攻关项目(GPK97-2-22A-1)通讯作者。 |
| |
| 摘 要： | 花粉管通道法是我国学者周光宇等在调查远缘杂交时提出的一种外源基因导入方法。本实验系用花粉管通道技术将C_4植物高粱总DNA(2D)导入C_3作物小麦甘麦8号(G8)和陇春13号(L13),结果在其后代中出现了广泛变异,并从中选育到了丰产、抗逆性强的小麦新品系89144(G8×2D)和89122(L13×2D)。连续几年的大田试验结果表明,其株形、抗锈性及经济产量显著优于其小麦亲本品种。为了进一步阐明这两个变异系光合性状优于其亲本
|
| 关 键 词： | 小麦 高梁 花粉管通道 δ13C值碳素固定途径 |
| 修稿时间： | 1998-11-23 |
STUDIES ON THE PHOTOSYNTHETIC CHARACTERISTICS OF WHEAT VARIANTS BY INTRODUCTION SORGHUM DNA |
| |
| WANG Xiao Juan,KONG Ying Zhen,WANG Ya Fu,PANG Bin Shuang,NI Jian Fu.STUDIES ON THE PHOTOSYNTHETIC CHARACTERISTICS OF WHEAT VARIANTS BY INTRODUCTION SORGHUM DNA[J].Journal of Molecular Cell Biology,2000,33(2):195-198. |
| |
| Authors: | WANG Xiao Juan KONG Ying Zhen WANG Ya Fu PANG Bin Shuang NI Jian Fu |
| |
| Institution: | WANG Xiao Juan
,KONG Ying Zhen
,WANG Ya Fu
;(The State Key Laboratory of Arid Agroecology, Lanzhou University, Lanzhou 730000);PANG Bin Shuang
,NI Jian Fu
;(Gansu Academy of Agricultural Sciences, Lanzhou 730070) |
| |
| Abstract: | Spring wheat Longchun No. 13 and Ganmai No. 8 were introduced by sorghum DNA via pollen tube pathway, and two stable progenies (89122 and 89144 respectively) were obtained through selection for several years.
The results of photosynthetic analysis showed that photosynthetic rate (P) and photosyn-thetic/transpiration rate (E/P) of 89122 and 89144 were mediated between their recipients and donor respectively, which demonstrated stronger photosynthetic efficiency than their receptors. However, the S'3 C values in assimilates from 89122, 89144 and their maternal wheat cultivars
G8 and L13 were approximately same, and they were significant different from sorghum. The chlorophyll content and the ratio of Chla/Chlb of them also showed that wheat cultivars and sorghum were different. It might demonstrated that the stronger photosynthetic efficiency of the progenies was due to their higher Calvin cycle metabolism efficiency rather than their carbon-fixation pathway difference. On the other hand, it also suggested that the distant genetic materials of the sorghum affected the photosynthetic characteristics of 89122 and 89144. |
| |
| Keywords: | Carbon-fixation Wheat Sorghum Pollen tube pathway C discriminating value |
| 本文献已被 CNKI 等数据库收录！ |
|
