A DNA fragment homologous to F1-ATPase beta subunit was amplified from genomic DNA of Methanosarcina barkeri. Indication of an archaebacterial F-type ATPase. |
| |
| Authors: | M Sumi M H Sato K Denda T Date M Yoshida |
| |
| Institution: | Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Yokohama. |
| |
| Abstract: | A 490 bp DNA fragment was amplified from Methanosarcina barkeri genomic DNA by the polymerase chain reaction (PCR) using oligonucleotide primers designed based on conserved amino acid sequences of the F1-ATPase beta subunits. The amino acid sequence deduced from the DNA sequence of this fragment was highly homologous to a portion of the F1-ATPase beta subunit. This indicates that this archaebacterium has a gene of F-type ATPase in addition to a gene of V-type ATPase. |
| |
| Keywords: | |
|
