Decreased surfactant protein B expression in mice derived completely from embryonic stem cells |
| |
| Authors: | Shumin Gao Xiaohui Wen Rongrong Yang Keqian Di Jun Tong Xiangyun Li |
| |
| Institution: | (1) College of Animal Science and Technology, Agricultural University of Hebei, Baoding, Hebei, 071001, China;(2) Cattle and Sheep Embryo Engineering Center of Hebei, Baoding, Hebei, 071001, China;(3) College of Chinese Traditional Veterinary, Agricultural University of Hebei, Baoding, Hebei, 071001, China |
| |
| Abstract: | ES mice that are derived completely from embryonic stem (ES) cells can be obtained by tetraploid embryo complementation. Many
neonate ES mice die because of respiratory distress, but it is not clear what contributes to the phenomenon. Using five microsatellite
DNA markers, we confirmed that our ES mice were completely derived from ES cells and contained no tetraploid component. The
neonatal ES mice that exhibited respiratory distress were tested for surfactant protein B (SP-B) expression by Western blotting.
These mice had no SP-B expression, and even apparently healthy adult ES mice had decreased SP-B levels and aberrant SP-B phenotypes.
These data suggest that the expression of SP-B protein is an important factor in the survival of ES mice to term and adulthood. |
| |
| Keywords: | ES mice Respiratory distress Pulmonary surfactant protein B Tetraploid embryo complementation |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
