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Analysis of gamma-carboxyglutamic acid via amino acid analysis.
Authors:D A Madar  R A Willis  K A Koehler  R G Hiskey
Institution:William Rand Kenan, Jr., Laboratories of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27514 USA
Abstract:Modifications of the analysis of protein-bound residues of γ-carboxyglutamic acid (Gla) via alkaline hydrolysis are presented. The method described allows easier sample manipulation than that heretofore required and insures quantitative recovery of hydrolyzed amino acids. A possible explanation of the shoulder which sometimes appears near Gla on some amino acid analyzers is presented.
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