Phosphorylation of hexoses in Streptomyces aureofaciens: Evidence that the phosphoenolpyruvate: Sugar phosphotransferase system is not operative |
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Authors: | Jana Novotná Zdenk Hoálek |
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Institution: | Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Prague 4, Czechoslovakia |
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Abstract: | Abstract Sugar phosphates are formed in cell-free extracts of Streptomyces aureofaciens RIA57 from glucose or fructose in the presence of phosphoenolpyruvate. In contrast to the phosphorylation by adenosine 5'-triphosphate the kinetics of formation of glucose 6-phosphate via phosphoenolpyruvate (PEP) is nonlinear. The product of fructose phosphorylation (only fructose 6-phosphate was determined by paper chromatography) and the absence of 1-phosphofructokinase indicate that fructose metabolism in S. aureofaciens does not proceed via the phosphoenolpyruvate:sugar phosphotransferase system (PTS). |
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Keywords: | Carbohydrate catabolism sugar phosphates glucose and fructose phosphorylation phosphoenolpyruvate hexokinases 1-phosphofructokinase |
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