Experimental Galactose Toxicity: Effects on Synaptosomal Phosphatidylinositol Metabolism |
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Authors: | Gerard Berry John R Yandrasitz Stanton Segal |
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Institution: | Division of Biochemical Development and Molecular Diseases, Children's Hospital of Philadelphia, and Departments of Pediatrics and Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, U.S.A. |
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Abstract: | Abstract Experimental galactose toxicity was induced by weaning rats onto an isocaloric 40% galactose diet. Synaptosomes were prepared from cerebra of rats at 2-9 weeks post-weaning and incubated with 33P]Pi and myo -2-3H]inositol in the presence or absence of 0.2 mM-acetylcholine. The acetylcholine-stimulated 33P]Pi labeling of phosphatidylinositol and the changes in amounts of phosphatidylinositol were similar in the normal and galactose-toxic rats; however, acetylcholine-stimulated myo -2-3H]inositol labeling of phosphatidylinositol was markedly decreased in the galactose-toxic rats. The impairment of acetylcholine-stimulated myo -2-3H]inositol incorporation into phosphatidylinositol observed after 2 weeks on the diet did not vary after more prolonged exposure to galactose. |
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Keywords: | Experimental galactose toxicity Synaptosome Acetylcholine Phosphatidylinositol Phospholipid effect |
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