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Experimental Galactose Toxicity: Effects on Synaptosomal Phosphatidylinositol Metabolism
Authors:Gerard Berry  John R Yandrasitz  Stanton Segal
Institution:Division of Biochemical Development and Molecular Diseases, Children's Hospital of Philadelphia, and Departments of Pediatrics and Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, U.S.A.
Abstract:Abstract Experimental galactose toxicity was induced by weaning rats onto an isocaloric 40% galactose diet. Synaptosomes were prepared from cerebra of rats at 2-9 weeks post-weaning and incubated with 33P]Pi and myo -2-3H]inositol in the presence or absence of 0.2 mM-acetylcholine. The acetylcholine-stimulated 33P]Pi labeling of phosphatidylinositol and the changes in amounts of phosphatidylinositol were similar in the normal and galactose-toxic rats; however, acetylcholine-stimulated myo -2-3H]inositol labeling of phosphatidylinositol was markedly decreased in the galactose-toxic rats. The impairment of acetylcholine-stimulated myo -2-3H]inositol incorporation into phosphatidylinositol observed after 2 weeks on the diet did not vary after more prolonged exposure to galactose.
Keywords:Experimental galactose toxicity  Synaptosome  Acetylcholine  Phosphatidylinositol  Phospholipid effect
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