Investigation of methods for measurement of radioactivity in tritiated DNA and applications to assays for DNA polymerase activity |
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Authors: | B K Schrier S H Wilson |
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Institution: | Behavioral Biology Branch, National Institute of Child Health and Human Development and Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014 USA |
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Abstract: | Methods for collection and counting of 3H-labeled DNA on nitrocellulose and glass fiber filters have been investigated. The findings are of potential importance in determining the amount of radioactivity in DNA as well as other macromolecules. The highest counting efficiencies were observed using glass fiber filters, NCS for dissolving DNA, and a toluene seintillation mixture for counting. However, glass fiber filters, even with large amounts of co-precipitant albumin, failed to collect all of low molecular weight (approx 185,000 daltons) DNA. Thus, in many applications nitrocellulose filters proved to be more advantageous. |
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