Anticoagulant activity of a sulfated chitosan |
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Authors: | Vongchan Preeyanat Sajomsang Warayuth Subyen Dumrat Kongtawelert Prachya |
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Affiliation: | Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand. pvongchan@hotmail.com |
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Abstract: | Chitin prepared from the shells of rice-field crabs (Somanniathelphusa dugasti) was converted into chitosan with a degree of acetylation of 0.21 and then sulfated with chlorosulfonic acid in N,N-dimethylformamide under semi-heterogeneous conditions to give 87% of water-soluble sulfated chitosan with degree of substitution (d.s) of 2.13. 1H NMR revealed the sulfate substitution at C-2, C-3 and C-6. Gel filtration on Sepharose CL-6B of the sulfated chitosan gave three fractions with average molecular weights of 7.1, 3.5, and 1.9 x 10(4). The three sulfated chitosan preparations showed strong anticoagulant activities, with the same mechanism of action observed for standard therapeutic heparin. |
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Keywords: | Chitosan Sulfation Somanniathelphusa dugasti Rice-field crab shell Anticoagulant activity |
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