Roles of ATP and NADPH in formation of the fe-s cluster of spinach ferredoxin |
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Authors: | Takahashi Y Mitsui A Fujita Y Matsubara H |
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Institution: | Department of Biology, Faculty of Science, Osaka University, Toyonaka, Osaka 560, Japan. |
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Abstract: | Ferredoxin (Fd) in higher plants is encoded by a nuclear gene, synthesized in the cytoplasm as a larger precursor, and imported into the chloroplast, where it is proteolytically processed, and assembled with the 2Fe-2S] cluster. The final step in the biosynthetic pathway of Fd can be analyzed by a reconstitution system composed of isolated chloroplasts and 35S]cysteine, in which 35S]sulfide and iron are incorporated into Fd to build up the 35S-labeled Fe-S cluster. Although a lysed chloroplast system shows obligate requirements for ATP and NADPH, in vitro chemical reconstitution of the Fe-S cluster is generally thought to be energy-independent. The present study investigated whether ATP and NADPH in the chloroplast system of spinach (Spinacia oleracea) are involved in the supply of 35S]sulfide or iron, or in Fe-S cluster formation itself. 35S]Sulfide was liberated from 35S] cysteine in an NADPH-dependent manner, whereas ATP was not necessary for this process. This desulfhydration of 35S]cysteine occurred before the formation of the 35S-labeled Fe-S cluster, and the amount of radioactivity in 35S]sulfide was greater than that in 35S-labeled holo-Fd by a factor of more than 20. Addition of nonradioactive sulfide (Na2S) inhibited competitively formation of the 35S-labeled Fe-S cluster along with the addition of nonradioactive cysteine, indicating that some of the inorganic sulfide released from cysteine is incorporated into the Fe-S cluster of Fd. ATP hydrolysis was not involved in the production of inorganic sulfide or in the supply of iron for assembly into the Fe-S cluster. However, ATP-dependent Fe-S cluster formation was observed even in the presence of sufficient amounts of 35S]sulfide and iron. These results suggest a novel type of ATP-dependent in vivo Fe-S cluster formation that is distinct from in vitro chemical reconstitution. The implications of these results for the possible mechanisms of ATP-dependent Fe-S cluster formation are discussed. |
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