| 疏水层析用于大规模纯化重组HBsAg的工艺研究 |
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| 引用本文： | 姚伟,李建民,毛晓燕,董继刚,张秋峰. 疏水层析用于大规模纯化重组HBsAg的工艺研究[J]. 微生物学免疫学进展, 2003, 31(3): 29-31 |
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| 作者姓名： | 姚伟 李建民 毛晓燕 董继刚 张秋峰 |
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| 作者单位： | 1. 兰州生物制品研究所,兰州,730046
2. 兰州大学生命科学学院,兰州,730000 |
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| 摘 要： | 应用疏水层析法从CHO细胞培养液中纯化HBsAg，每根制备柱每次可处理细胞收液350L，在适宜的上样流速和层析温度条件下，层析后可去除96％的杂蛋白，再经超速离心和凝胶过滤层析，可获HBsAg纯品。经检定，HPLC纯度高于95％，其余各项检定指标均符合《中国生物制品规程》要求。结果表明，此方法纯化效率高、处理样品量大、成本低，适于大规模生产。
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| 关 键 词： | 疏水作用层析 纯化 重组乙肝表面抗原 |
| 文章编号： | 1005-5673(2003)03-0029-03 |
| 修稿时间： | 2002-12-06 |
The process study for the large scale purification of recombinant HBsAg by Hydrophobic Interaction Chromatography |
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| YAO Wei,LI Jian-min ,MAO Xiao-Yan ,et al.. The process study for the large scale purification of recombinant HBsAg by Hydrophobic Interaction Chromatography[J]. Progress In Microbiology and Immunology, 2003, 31(3): 29-31 |
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| Authors: | YAO Wei LI Jian-min MAO Xiao-Yan et al. |
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| Affiliation: | YAO Wei,LI Jian-min 1,MAO Xiao-Yan 2,et al. |
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| Abstract: | Recombinant HBsAg which obtained from the culture fouid of CHO cell was purified by Hydrophobic Interaction Chromatography(HIC).One column can purify about 350L culture fluid.Under the suitable temperature and flow rate,96% of impure proteins was eliminated after HIC.The purificatory HBsAg was obtained by zonal centrifugation and gel-filtration chromatography(GFC)at last.The purity of HPLC was over 95%.The other results of the test were qualified.The results indicate that HIC is suitable for the large scale purification of recombinant HBsAg. |
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| Keywords: | Hydrophobic interaction chromatography(HIC) Purification Recombinant rHBsAg |
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