Maturation ameloblasts of the porcine tooth germ do not express amelogenin |
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Authors: | K Wakida Norio Amizuka Chikage Murakami Takahiro Satoda Makoto Fukae James P Simmer Hidehiro Ozawa Takashi Uchida |
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Institution: | (1) Department of Oral Anatomy, Hiroshima University School of Dentistry, Kasumi 1–2-3, Minami-ku, Hiroshima 734-8553, Japan e-mail: wakida@ipc.hiroshima-u.ac.jp Tel.: +81-82-257-5624 Fax: +81-82-257-5689, JP;(2) Department of Oral Anatomy, Niigata University School of Dentistry, Gakkochou 2-5274, Niigata 951-8154, Japan, JP;(3) Department of Biochemistry, School of Dental Medicine, Tsurumi University, Tsurumi 2–1-3, Yokohama 230-8501, Japan, JP;(4) Department of Pediatric Dentistry, School of Dentistry, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78284-7888, USA, US |
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Abstract: | Amelogenins are the most abundant constituent in the enamel matrix of developing teeth. Recent investigations of rodent incisors
and molar tooth germs revealed that amelogenins are expressed not only in secretory ameloblasts but also in maturation ameloblasts,
although in relatively low levels. In this study, we investigated expression of amelogenin in the maturation stage of porcine
tooth germs by in situ hybridization and immunocytochemistry. Amelogenin mRNA was intensely expressed in ameloblasts from
the differentiation to the transition stages, but was not detected in maturation stage ameloblasts. C-terminal specific anti-amelogenin
antiserum, which only reacts with nascent amelogenin molecules, stained ameloblasts from the differentiation to the transition
stages. This antiserum also stained the surface layer of immature enamel at the same stages. At the maturation stage, no immunoreactivity
was found within the ameloblasts or the immature enamel. These results indicate that, in porcine tooth germs, maturation ameloblasts
do not express amelogenins, suggesting that newly secreted enamel matrix proteins from the maturation ameloblast are not essential
to enamel maturation occurring at the maturation stage.
Accepted: 14 January 1999 |
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