| 巴西橡胶树SSR遗传图谱的构建 |
| |
| 引用本文： | 冯素萍,李维国,于飞,王静毅,武耀廷.巴西橡胶树SSR遗传图谱的构建[J].遗传,2010,32(8):857-863. |
| |
| 作者姓名： | 冯素萍 李维国 于飞 王静毅 武耀廷 |
| |
| 作者单位： | 1. 中国热带农业科学院热带作物生物技术研究所, 海口 571101;
2. 中国热带农业科学院橡胶研究所, 儋州 571737;
3. 琼州学院, 五指山 572200;
4. 海南大学农学院,海口 570228 |
| |
| 基金项目： | 中央级公益性科研院所基本科研业务费项目 |
| |
| 摘 要： | 以热研88-13×IAN873的94个F1群体为试材, 利用简单序列重复(Simple sequence repeat, SSR)标记, 采用FsLinkageMAP 1.0软件, 构建了巴西橡胶树热研88-13×IAN873的遗传连锁图谱。从441对SSR引物中筛选出160对具有多态信息的引物, 在分离群体中共检测到206个多态性位点, 176个位点用于遗传图谱的构建; χ2检验结果显示, 有147个位点符合1:1分离比例, 有12个符合1:2:1分离比例, 有17个符合1:1:1:1的分离比例, 共有13个偏分离位点, 偏分离率低(7.38%); 91个SSR位点被分为18个连锁群, 覆盖橡胶树基因组1 937.06 cM, 每个连锁群包含2～16个位点, 标记间的平均距离为21.29 cM。
|
| 关 键 词： | 巴西橡胶树 SSR标记 遗传图谱 |
| 收稿时间： | 2009-10-16 |
Construction of genetic linkage map for rubber tree(Hevea brasiliensis)based on SSR markers |
| |
| FENG Su-Ping,LI Wei-Guo,YU Fei,WANG Jing-Yi,WU Yao-Ting.Construction of genetic linkage map for rubber tree(Hevea brasiliensis)based on SSR markers[J].Hereditas,2010,32(8):857-863. |
| |
| Authors: | FENG Su-Ping LI Wei-Guo YU Fei WANG Jing-Yi WU Yao-Ting |
| |
| Institution: | 1. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou 571101, China;
2. Rubber Cultivation Research Institute, Chinese Academy of Tropic Agricultural Science, Danzhou 571737, China;
3. Qiongzhou University, Wuzhishan 572200, China;
4. Agricultural College, Hainan University, Haikou 570228, China |
| |
| Abstract: | Out of 260 polymorphic loci screened from a total of 441 pairs of EST-SSR and genomic-SSR primers, 176 were used for constructing the genetic map of rubber tree (Hevea brasiliensis) by an F1 segregating population including 94 progenies from the cross Reyan 88-13×IAN873. Chi-square test carried out on the polymorphic loci used in constructing the map showed that 147 loci followed a segregation ratio of 1:1 and 12 loci followed a ratio of 1:2:1 and 17 loci followed a ratio of 1:1:1:1. Only 13 (7.38%) loci were distorted from the Mendelian ratio. The genetic linkage map consisted of 91 marker loci in 18 linkage groups and covered 1 937.06 cM with an average genetic distance of 21.29 cM between adjacent markers. The largest linkage group consisted of 16 marker loci, while the smallest one contained only 2 marker loci. |
| |
| Keywords: | rubber tree (Hevea brasiliensis) SSR markers genetic lingkage map |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《遗传》浏览原始摘要信息 |
| 点击此处可从《遗传》下载免费的PDF全文 |
|
