Glycollate production and excretion byAlcaligenes eutrophus |
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Authors: | G. A. Codd B. Bowien H. G. Schlegel |
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Affiliation: | (1) Institut für Mikrobiologie der Universität, Grisebachstr. 8, D-3400 Göttingen, Federal Republic of Germany;(2) der Gesellschaft für Strahlen- und Umweltforschung mbH, Grisebachstr. 8, D-3400 Göttingen, Federal Republic of Germany;(3) Present address: Department of Biological Sciences, University of Dundee, DD1 4HN Dundee, UK |
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Abstract: | Autotrophic cultures of the facultative chemolithotrophAlcaligenes eutrophus have been found to excrete glycollate. This excretion was greatly stimulated by the incorporation of up to 20% (v/v) oxygen in the hydrogen used for gassing. The stimulatory effect of oxygen was prevented by the addition of 10% (v/v) CO2 to the gassing mixture. Glycollate excretion only in the presence of oxygen was increased by the addition of 2-pyridyl-hydroxymethane sulphonic acid (HPMS), an inhibitor of glycollate oxidation, indicating that glycollate formation itself was stimulated by oxygen. No glycollate excretion by cultures grown heterotrophically on pyruvate was detected, either in the absence or presence of HPMS, under heterotrophic or autotrophic conditions.Extracts from autotrophic cells showed phosphoglycollate phosphatase and glycollate oxidoreductase activities, which were considerably lower in extracts prepared from pyruvate- or fructose-grown (heterotrophic) cells. The increase in activity of both enzymes upon cell transfer from heterotrophic to autotrophic growth was prevented by chloramphenicol and resembled the induction ofd0ribulose 1,5-diphosphate carboxylase under the same conditions.Abbreviations DTE dithioerythritol - EDTA ethylenediamine tetraacetate - HPMS 2-pyridyl-hydroxymethane sulphonie acid - RuDP d-ribulose 1,5-diphosphate |
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Keywords: | Glycollate excretion Phosphoglycol-late phosphatase Glycollate oxidoreductase font-variant:small-caps" >d-Ribulose 1,5-diphosphate carboxylase Alcaligenes eutrophus |
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