禽流感病毒感染食蟹猴肺组织内抗原提呈细胞亚型分析

目的分析禽流感病毒感染的食蟹猴肺组织内抗原提呈细胞的不同亚型。方法收集中国医学科学院实验动物研究所病理室禽流感病毒H5N1亚型感染的食蟹猴肺组织蜡块标本，观察其病理学变化及运用免疫组织化学的方法进一步分析CD68、CD14及S-100在感染禽流感病毒食蟹猴肺组织中的表达。结果禽流感病毒感染的肺组织内肺间隔增宽伴巨噬细胞为主的炎细胞浸润，肺泡腔内可见血浆蛋白及纤维素渗出。部分肺泡间隔断裂、巨噬细胞及淋巴细胞浸润。肺组织内H5N1病毒抗原染色呈阳性。肺间隔及肺泡内可见大量CD68阳性细胞，局部可见散在CD14和S-100阳性细胞。结论禽流感病毒感染的食蟹猴肺组织内抗原提呈细胞主要以巨噬细胞为主。

Analysis of Antigen Presenting Cell Cynomolgus Macaque Infected Subtypes in the Lung Tissue of with Avian Infuenza Virus

Objective To analyze the subtypes of antigen presenting cells in the lung tissue of cynomolgus macaque infected with avian influenza virus. Method Paraffin-embedded blocks of lung tisse of cynomolgus macaque inoculated with avian influenza virus were obtained from the Department of Pathology of this Institute. The distribution and expression of CD68, CD14 and S-100 proteins in infected lung tissue were analyzed by histopathology and immunohistochemsitry. Results The interstitial thickening and macrophage infiltration were observed in the lung tissue of the monkeys infected with HSN1 avian influenza virus. Exudation of protein-rich edema fluid and fibrin were also found in the alveolar cavities. Focal alveoli were destroyed and many macrophages infiltrated in the alveolar septa and alveolar cavities. Strong HSN1 viral antigen staining was observed in the infected lung tissue. Diffuse CD68 positive cells were found in alveolar septa and alveolar cavity. In contrast, only rare CD14 and S-100 positive cells were observed in the lung tissue. Conclusion Macrophages are the main antigen presenting cells in the lung tissue of cynomolgus macaque infected with avian influenza virus.