去势大鼠新鲜、冷冻卵巢移植后激素代谢变化

目的观察去势大鼠经新鲜、冷冻卵巢移植后体内激素代谢变化情况。方法24只去势大鼠经右下腹皮下移植新鲜卵巢（3 d之内乳鼠卵巢）和经过冻存培养后的卵巢（3 d之内乳鼠卵巢）后,检测移植前、移植后1个月2、个月的三碘甲腺原氨酸（T3）、甲状腺素（T4）、促甲状腺激素（TSH）、睾酮（T）、雌二醇（E2）、孕酮（P）、黄体生成素（LH）及卵泡生成素（FSH）。结果冻存卵巢移植前与新鲜卵巢移植前比较：T值升高,有显著性差异（P〈0.01）;冻存卵巢移植后1个月与新鲜卵巢移植后1个月比较差异无显著性（P〉0.05）;冻存卵巢移植后2个月与新鲜卵巢移植后2个月比较：E2升高明显,差异具有显著性（P〈0.01;新鲜卵巢移植后2个月与移植前比较：T4,T,E2,P,LH均有明显升高（P〈0.01;P〈0.05）;冻存卵巢移植后2个月与移植前比较：E2,P增高明显（P〈0.01;P〈0.05）。结论冷冻卵巢移植较新鲜卵巢移植功能恢复明显。

Hormonal Metabolic Changes in Fresh and Frozen Ovary Transplantation in Castrated Rats

Objective To observe of hormonal metabolic changes in fresh and frozen ovary transplantation in castrated rats.Method The castrated rats were subcutaneously transplanted with fresh or frozen ovary（neonatal rat within three days） in the right lower abdomen.The levels of T3,T4,TSH,T,E2,P,LH and FSH in the rats were measured before transplantation and at the first,second month after transplantation.Result The T level in the frozen ovary transplantation group was significantly higher compared with that of the fresh ovary transplantation group（P〈0.01）.The hormone indexes of the frozen ovary transplantation group were not significantly different compared with those in the fresh ovary transplantation group at the first month after transplantation（P〉0.05）;The E2 of the frozen ovary transplantation group was significantly higher compared with that in the fresh ovary transplantation group at the second month after transplantation（P〈0.01）.The levels of T4,T,E2,P,LH of the fresh ovary transplantation group were significantly higher in comparison with those in the rats before transplantation at the second month after operation（P〈0.05;P〈0.01）.The levels of E2 and P of the frozen ovary transplantation group at the second month after operation were significantly higher compared with those before operation （P〈0.05;P〈0.01）.Conclusions The hormone level of the rats with frozen ovary transplantation are significantly elevated in comparison with the rats with fresh ovary transplantation.