| 小鼠异位心脏移植模型的改良 |
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| 引用本文： | 冯嘉瑜,;贺伟峰,;朱晓星,;宋波,;杨康,;张银甫,;黄赤兵.小鼠异位心脏移植模型的改良[J].中国实验动物学杂志,2008(3):68-71. |
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| 作者姓名： | 冯嘉瑜 ;贺伟峰 ;朱晓星 ;宋波 ;杨康 ;张银甫 ;黄赤兵 |
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| 作者单位： | [1]第三军医大学新桥医院泌尿外科,重庆400037; [2]第三军医大学西南医院全军泌尿中心,重庆400038; [3]第三军医大学西南医院胸心外科,重庆400038; [4]第三军医大学西南医院烧伤研究所,重庆400038 |
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| 基金项目： | 第三军医大学校管课题资助项目（2004D109）,国家自然科学基金资助项目（项目批准号：30571863）. |
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| 摘 要： | 目的探讨小鼠心脏移植模型的建立与改良。方法供体采用BLAB／c小鼠，受体采用C57小鼠。用24GACuff管法套扎供心肺动脉与颈外静脉，用自制Cuff管套扎供心升主动脉与颈总动脉。结果供心冷缺血时间少于15min，手术时间大大缩短，成功率明显提高。结论改进后的模型显著降低了手术难度，可用于移植免疫的各种研究。
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| 关 键 词： | 小鼠 异位 心脏移植 |
Improved Technique of Cervical Heterotopic Heart Transplantation in Mice by Self-made Cuff |
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| Institution: | FENG Jia-yu, HE Wei-feng, ZHU Xiao-xing , S0NG Bo ,YANG Kang , ZHANG Yin-fu , HUANG Chi-bing (1. Department of Urology, Xinqiao hospital, Third Military Medical University, Chongqing 400037, China; 2. Department of Urology, Southwest hospital, Third Military Medical University, Chongqing 400038, China; 3. Department of cardiothoracic surgery, Xinqian hospital, Third Military Medical University, Chongqing 400038, China; 4. Bum research insititute of Southwest Hospital, Third Military Medical University, Chongqing 400038, China ; ) |
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| Abstract: | Objective To explore the establishment and improved cuff technique of cervical heterotopic heart transplantation in mice. Methods We connected the recipient＇s external jugular vein with aorta by using a 24GA Cuff and the recipient＇s common carotid artery whit the pulmonary aorta by using self-made Cuff. Result The modified methods results in the cold ischemia time less than 15 minutes. Conclusions The modified methods are easier and more practical than before and can be used to research mechanisms of immunity of transplantation. |
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| Keywords: | Mouse Heterotopic Heart transplantation |
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