BALB/c小鼠遗传质量检测的新方法

目的比较随即扩增多态性方法（RAPD）、微卫星方法（STR）与生化标记方法对近交系小鼠遗传质量检测的差异,为近交系动物遗传质量控制提供一种分子生物学方法。方法提取近交系小鼠BALB/c基因组DNA,用6条RAPD引物和20对STR引物对其进行PCR扩增,用生化标记法检测13个位点。结果在6条RAPD引物中,引物2（p2）、引物3（p3）、引物5（p5）和引物6（p6）这四条引物扩增的条带出现差异,表现为不同的RAPD图谱;在20对STR引物中,引物2、4、10和11,这四对引物扩增的条带出现差异,表现为不同的STR图谱;13个生化标记位点中,过氧化氢酶-2（Ce-2）等6个生化位点发现杂合基因。结论RAPD和STR可用于验证生化标记方法的实验结果,并用于保证近交系动物的遗传质量。

A New Method for Monitoring the Genetic Quality of BALB/c Mice

Objective In order to explore a new method for monitoring the genetic quality of laboratory animals, a comparison of RAPD map and STR map with the biochemical labeling in monitoring inbred BABL/e mouse was carried out. Methods DNA was extracted from the tail of inbred BALB/c mice. 6 pairs of RAPD primers and 20 pairs of STR primers were used to amplify the mouse DNA. 13 loci of biochemical analysis were used to monitor the quality of inbred BALB/c mouse. Results The amplified band patterns were obviously different in the products of 4 RAPD primers （p2, p3,195 and p6） and 4 STR primers （2,4,10,11）,and 6 loci depicted by biochemistry were different in the inbred BALB/c mice. Conclusion The results indicate that to a certain extent the results of RAPD and STR are comparable with that of biochemical labeling, and RAPD and STR can be used to monitoring the genetic quality of inbred laboratory animals.