ConA引起小鼠肝损伤实验研究

目的探讨conA引起免疫性肝损伤机实验条件。方法测定两个浓度，不同时间点ConA尾静脉注射后小鼠转氨酶水平及肝、脾病理变化。结果15mg／kgConA尾静脉注射后8h，血清转氨酶升高，但病检无明显改变；20mg／kgConA尾静脉注射，脾指数6h达峰值，10h肝脏病理变化显著，转氨酶水平达峰值。结论20mg／kgConA小鼠尾静脉注射6h后脾病变达高峰，10h可引起显著性肝损害。

Liver Injury in Mice Induced by ConA

Objective To explore the optimal detective conditions of different indexes on ConA induced immune liver injury in mice. Methods Determination the releasing of transaminases and the pathologic change after mouse vena caudalis injection of ConA with different concentrations and at different time. Results The transaminases heightened at 8h after ICR mouse vena caudalis injection by ConA 15mg/kg, though nuance on pathologic change. When vena caualis injection of ConA 20/kg, the spleen index reached the zenith within 6 hours; while at 10 hour after injection, the hepatic pathology became distinct as well as the transaminases. Conclusion The spleen pathologic change take place within 6 hours after 20 mg/kg ConA vena caualis injection on ICR mouse, while 10 hour could rise the most conspicuous liver injury.