降钙素基因相关肽肽段的抗原性和免疫原性

用固相合成法合成了有关降钙素基因相关肽ＣＧＲＰ五个肽段。它们的氨基酸顺序分别为：ＣＧＲＰ－１（２４－３７）；ＣＧＲＰ－２（１４－３７）；ＣＧＲＰ－３（９－２３）；ＣＧＲＰ－４（１－２３）和ＣＧＲＰ－５（１－１３）。用酶标方法测定了它们的抗原性，发现ＣＧＲＰ－２和ＣＧＲＰ－１能够很好地与抗ＣＧＲＰ抗血清结合。将ＣＧＲＰ－１；ＣＧＲＰ－３和ＣＧＲＰ－５分别免疫兔子，获得的抗血清分别与ＣＧＲＰ进行反应，发现只有抗ＣＧＲＰ－１的抗血清能够与ＣＧＲＰ很好地结合。因此，可以推测ＣＧＲＰ的羧端部分是其免疫活性部位。

The Antigenicity and Immunogenicity of Fragments of CGRP

Calcitonin gene-related peptied(CGRP) and its five fragments CGRP-1,CGRP-2,CGRP-3,CGRP-4 and CGRP-5,corresponding respcctively to residues 24-37,14-47,923,1-23 and 1-13, were synthesized by solid-phase method, and antibodies raised to CGRP, CGRP-1,CGRP-3 and CGRP-5 were obtained from rabbits. Antigenicity of CGRP and the five fragments was measured by ELISA, and we found that the ability of the series of peptides to bind CGRP antibodies was CGRP>CGRP-2>CGRP-1>>CGRP-3>CGRP-4 >CGRP-5.We also used CGRP to react respectively with antisera raised from CGRP,CGRP-1,CGRP-3 and CGRP-5 , and found that immunogemcity of the different pepties was CGRP≈CGRP-1>>CGRP-3 and CGRP-5. It appears that for these five fragrnents of CGRP, the properties of immunogenicity and antigenicity run parallel. That is CGRP-1 is the most immunogenic in producing a larger antibody titer to the parent peptide. This finding suggests that the C-terminal region of CGRP is the position of immunogenic activity.