Deinococcus radiodurans DNA increases the radiation resistance of Escherichia coli |
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| Authors: | G V Dalrymple T J Lynch J W Hardin |
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| Institution: | Nuclear Medicine Service, John L. McClellan Memorial Veterans Hospital, Little Rock, Arkansas. |
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| Abstract: | A genomic DNA library of Deinococcus radiodurans DNA has been prepared using the plasmid vector pBR322. The recombinant plasmid was used to transform a more radiation-sensitive organism, Escherichia coli RR1. Following selection of transformed organisms by their ability to grow on ampicillin, radiation-resistant organisms were selected by irradiation with 137Cs gamma radiation. Increased radiation resistance correlates with the presence of a 3-kb fragment of DNA in these cells which is derived from D. radiodurans. |
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