"In vivo" (35S)-methionine interaction with rat-liver DNA and the effect of chemical carcinogens |
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| Authors: | D Kanduc E Quagliariello |
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| Institution: | Dipt. di Biochimica e Biologia Molecolare, Facoltà di Scienze, Università di Bari, Italy. |
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| Abstract: | 1. After intraperitoneal administration of (35S)methionine (25 mg, 1.6 mCi/kg), detectable amount of radioactivity resulted associated to rat-liver DNA: the interaction reached the maximum value (about 18 pmol/mumol DNA P) by 2 h after administration of radioactive aminoacid. 2. The (35S)-binding was inhibited by the hepatocarcinogenic ethionine and dimethylnitrosamine, and was stimulated by the non-hepatocarcinogenic methylnitrosourea. 3. Hplc analysis of (35S)DNA enzymic digest evidenced two radioactive compounds, the UV behaviour of which is reported. |
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