CWE纯系小鼠脑组织发育过程中细胞癌基因及其表达研究

 本文以Ha-ras.c-fos、c-myc、v-erbB、mos五种癌基因片段为分子探针,应用斑点杂交技术,对CWE纯系小鼠脑组织发育的四个时期(胚胎期、新生期、性成熟期、体成熟期)的RNA进行分子杂交分析。发现:在实验各期中这几种癌基因的表达有较明显的变化。Ha-ras、c-fos、c-myc基因的表达在胚胎期和新生期脑组织中较高;v-erbB基因的表达在性成熟期脑组织中较高;mos基因在实验各期均无表达。DNA斑点杂交结果:小鼠脑组织发育各期均未观察到癌基因扩增现象。Southern杂交结果:CWE小鼠发育的各期均显示:5.4kb、3.4kb、1.8kb、1.0kb四条c-fos基因区带;7.4kb、4.5kb、3.4kb三条c-myc基因区带。结果表明:不同细胞癌基因在小鼠胚胎发育和胚后发育中有不同的表达。

The Expression of Cellular Oncogenes During Brain Development of CWE Mouse

The expression of 5 oncogenes was examined in 4 developmental stages (embryo, newborn, sexual maturity and adult)of CWE mouse brain tissue.Brain RNA was detected with Ha-ras, c-fos, c-myc v-erb B and mos oncogene probes by dot blot hybridization. The expression of these oncogenes was demonstrated.Ha-ras, c fos, c-myc were expressed at high level in the embryo and newborn brain, but erbB showed higher expression at the stage of sexual maturity. In contrast, mos oncogene showed no expression at all.At the same time the DNA of brain tissue was studied by dotblot hybridization, the result showed that there was no oncogene amplification in any developmental stage; by Southern blot transfer of DNA digested with BamH I, 4 c-fos oncogene bands of 5.4, 3.4, 1.8, 1.0kb and 3 c-myc oncogene bands of 7.4, 4.3, 3.4kb were observed, but there was no difference in these 4 stages.