Apolipoprotein-J prevention of fetal cardiac myoblast apoptosis induced by ethanol |
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Authors: | Li Yangxin Sagar Madi Bidya Wassler Michael Shelat Harnath Geng Yong-Jian |
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Institution: | Texas Heart Institute and The University of Texas Health Science Center, Houston, TX 77030, USA. |
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Abstract: | Over-consumption of ethanol (EtOH) represents a major health problem. This study was to test the cytotoxicity of EtOH in cardiac stem cells or myoblasts, and the potential protective effect of apolipoprotein-J (ApoJ), a stress-responding, chaperone-like protein in high-density lipoprotein, on EtOH-injured cardiac myoblasts. In culture, EtOH-exposed canine fetal myoblasts underwent apoptosis in a concentration- and time-dependent manner. Expression ApoJ by cDNA transfection markedly reduced EtOH-induced apoptosis in the cells. ApoJ expression also restored partially the mitochondrial membrane potential and prevented the release of cytochrome-c from mitochondria into cytoplasma. Thus, ApoJ serves as a cytoprotective protein that protects cardiac stem cells against EtOH cytotoxicity. |
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Keywords: | Apoptosis Stem cells Ethanol Cytochrome-c Mitochondria Lipoprotein Heart |
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