The Na+,K+/H+ -antiporter Nha1 influences the plasma membrane potential of Saccharomyces cerevisiae |
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Authors: | Kinclova-Zimmermannova Olga Gaskova Dana Sychrova Hana |
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Institution: | Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic. |
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Abstract: | There are three different sodium transport systems (Ena1-4p, Nha1p, Nhx1p) in Saccharomyces cerevisiae. The effect of their absence on the tolerance to alkali-metal cations and on the membrane potential was studied. All three sodium transporters were found to participate in the maintenance of Na+, Li+, K+ and Cs+ homeostasis. Measurements of the distribution of a fluorescent potentiometric probe (diS-C3(3) assay) in cell suspensions showed that the lack of all three transporters depolarizes the plasma membrane. The overexpression of the Na+,K+/H+ antiporter Nha1 resulted in the hyperpolarization of the plasma membrane and consequently increased the sensitivity to Cs+, Tl+ and hygromycin B. This is the first evidence that the activity of a Na+,K+/H+ antiporter could play a role in the homeostatic regulation of the plasma membrane potential in yeast cells. |
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Keywords: | Saccharomyces cerevisiae salt tolerance hygromycin B membrane potential Na+ K+/H+ antiporter diS-C3(3) assay |
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