Involvement of hydroxyl radical in NAD(P)H oxidation and associated oxygen reduction by the granule fraction of human blood polymorphonuclears. |
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Authors: | C Auclair M Torrès J Hakim |
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Institution: | Laboratoire Central d''Immunologie et d''Hématologie de l''Hôpital Bichat U 160 (INSERM), ERA 573 (CNRS), Université Paris VII 170, boulevard Ney - 75877 Paris Cedex 18 - France |
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Abstract: | The cyanide-insensitive NAD(P)H oxidase activities have been measured in particulate fractions isolated from resting or zymosan-stimulated polymorphonuclear leukocytes. The particulate fraction was primarily composed of granules. The activities were measured both in the presence and absence of Mn++. It was found, in all experiments, that hydroxyl radical scavengers such as Tris, benzoate or mannitol, were powerful inhibitors of the NAD(P)H oxidase activities. This was taken as evidence for the involvement of hydroxyl radical as an intermediate in the aerobic oxidation of both NADH and NADPH. Possibles sources of hydroxyl radical are suggested, but none of them is demonstrated. |
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Keywords: | PMN human polymorphonuclear blood cells superoxide anion radical hydrogen peroxide OH· hydroxyl radical singlet molecular oxygen KRP calcium-free Krebs Ringer Phosphate |
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